Composite
Edd-Device

Part:BBa_K1840005:Design

Designed by: Julia Anna Adrian   Group: iGEM15_NTNU_Trondheim   (2015-09-18)


Device: TT-edd-mCherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As a promotor sequence, we assumed all DNA in front of the edd gene up to the next gene, coding for gap-1. Since, it is encoded on the opposite strand, the is the possibility, that edd functions as a promotor for this gene as well. Therefore the double terminator was needed.


Source

The double terminator was already registered in the iGEM catalogue. The edd comes from the genome of Pseudomonas putida. The mCherry is codon optimized for expression in Pseudomonas.

References